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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in several tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was uncovered to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant purpose in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two further splice variants were described but in depth nucleotide sequence details is just not however readily available.
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Blocking peptides are peptides that bind particularly on the concentrate on antibody and block antibody binding. These peptide generally has the epitope recognized through the antibody.
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This guideline outlines further achievable optimization of mobile and immunoassay parameters to make certain the absolute best final results are...
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This guide outlines even more doable optimization of cellular and immunoassay parameters to be sure the absolute best final results are...
Antibodies certain to the blocking peptide not bind to the epitope to the target protein. By comparing the staining with the blocked antibody compared to the antibody by itself, one can see which staining is distinct.
Concentrate on Information Cyclin E belongs on the hugely conserved cyclin family members, whose members are characterised by a extraordinary periodicity in protein abundance from the mobile cycle. Cyclins operate as regulators of CDK kinases. Various cyclins show distinctive expression and degradation designs which contribute into the temporal coordination of every mitotic function. Cyclin E forms a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S changeover. This protein accumulates at the G1-S phase boundary and is particularly degraded as cells progress by S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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